Immobilization of Spore Laccase: Comparative Studies and Carriers Selection

Abstract:

The comparative studies for immobilization of spore laccase were carried out using 19 different methods and 12 carriers. The optimal carrier and method were determined by the activity yield，reusability，and cost of the immobilized spore laccase. The ionic bonding method using DEAE-cellulose as the carrier was selected as the best method. The strong diffusion limitations were observed when the spore laccase was immobilized by embedding method. The microspheres might dissolve and swell if semi-embedding method was used. The small pore size made it difficult for porous materials to immobilized spore laccase. The excellent immobilization properties were achieved when the spores were covalently bound to the carriers using carbodiimide as the crosslinker. It was thus recommended as an alternative method.