γ-CD Catalyzed from Maize Starch by Dual Enzymatic Reaction

Abstract:

Theconjunction of 4α-glycosyltransferase（4αGTase） with cyclodextrin glycosyltransferase（CGTase） were screened for their ability ofproducing γ-cyclodextrin（CD） usingmaize starch as substrate. The studies on the influence factors showed that 4αGTase should be added first，followed byCGTase. The optimal conditions for γ-CD production was as following：4U of 4αGTase and 8U ofCGTase per gram of substratewere used to convert maize starch（5%，w/v） into γ-CD with the reaction time of 30 h. the subsequent yield of γ-CD was 12.83%，which was 76.7% higher than the control. This study has potential value for the industrial production of γ-CD.