Study on High Performance Liquid Chromatography Method for Determination of Enzymatic Preparation of L-Tryptophan

Abstract:

High performance liquid chromatography method for determination of enzymatic Preparation of L-tryptophan（L-Trp） were studied. The results showed that the optimal parameter were 0.05 mmol/L potassium dihydrogan phosphate - methanol （30∶70） as the mobile phase，the detection wavelength by 265 nm，the flow rate of 1.3 mL/min，separated on a C18 column and 38 ℃ column temperature. The recoveries ranged from 92.00%~110.00%，and the Serine added had no interference to determination of L-tryptophan. The L-Trp in fermentation liquid was separated effectively in these conditions.