Research on Antibacterial Activity of OVT under Heat Treatment

Abstract:

Ovotransferrin， an iron-binding globulin， had a broad spectrum of antibacterial activity. Its antibacterial activity was closely related with its structure. In this study， the impact of heat treatment on the structure and antibacterial activity of ovotransferrin was studied， and the change mechanism of the antibacterial effect was revealed through the analysis of its secondary structure and hydrophobic effect. Results showed that the antibacterial activity of ovotransferrin on Escherichia coli and Salmonella was decreased with the increase of treatment temperature. The structure of ovotransferrin got changed after the heat treatment， the contents of α-helix and random coil of ovotransferrin were decreased due to the high temperature， meanwhile the contents of β-sheet and the β-turn were increased. Besides， surface hydrophobicity of ovotransferrin was significantly increased. As processing time extension， the antibacterial activity of ovotransferrin had no significant change at the same temperature.