Preparation and Characterization of Nano-Silver Loaded Fibroin/Alginate Sponge

Abstract:

This study aimed to fabricate a novel Ag nano-particles（AgNPs） loaded biologic dressing based on silk fibroin（SF） and sodium alginate（SA）. AgNPs were first synthesized in the stabilizer，SF solution，under UV irradiation，and after the addition of SA，were then treated by CaCl2/ethanol /water（molar ratio，1∶71∶77） for 3 h. After rinsing and freeze-drying，AgNPs loaded SF/SA composite sponge（AgNPs-SF/SA） was obtained. Transmission electron microscopy observation（TEM） showed that AgNPs were evenly distributed in the SF solution with a mean diameter of 5.03 nm. Scanning electron microscopy（SEM） images showed that compared with pure SF sponge，AgNPs-SF/SA had a linked porous structure. Addition of SA improved the water absorption and retention ability of the sponge and the synergistic effect of SF and SA increased the water vapor transmission rate. K-B disk diffusion and bacterial solution culture methods indicated that AgNPs-SF/SA possessed a higher and more continuous anti-bacterial potential than the positive control，AQUACEL?誖 Ag，against P. aeruginosa and S. aureus. In conclusion，the excellent physical and anti-bacterial properties demonstrated that AgNPs-SF/SA could be a novel anti-bacterial biologic dressing for prevention and treatment of wound infection.