Optimization of Fermentation Medium for the Production of Pigments by Marine Bacteria Planococcus rifietoensis

Abstract:

Based on monofactorial experiments，Plackett-Burman（PB） design and response surface methodology（RSM） were applied to optimize 8 medium components for the production of pigments by marine bacteria strain Planococcus rifietoensis. Results showed that optimal carbon and nitrogen sources of the medium were glucose and peptone，respectively，and glucose，yeast extract and MgSO4·7H2O had major effects on the production of pigments. The optimal culture medium was comprised of glucose 9.78 g/L，peptone 8.00 g/L，yeast extract 2.05 g/L，MgSO4·7H2O 8.17 g/L，Na2HPO4 0.1 g/L，FeSO4·7H2O 0.005 g/L，NaCl 10 g/L and CaCl2 0.1 g/L. The incubation time was 54 hours. Under these conditions，the optical density（OD） of pigments reached to 0.984，which was 245% higher than that before optimization.