Physiological Mechanism of Transcription Factor Asg1p for the Regulation of Robustness of Candida glabrata

Abstract:

To analyze the effect of transcription factor CgAsg1p on the robustness of Candida glabrata，the mutant strain Cgasg1?驻 was constructed and the cell growth，gene transcriptional level and the ability of resisting various stresses were investigated. Compared to the wild-type strain C. glabrata ATCC 2001，the mutant strain Cgasg1?驻 lost the ability of surviving in the medium at pH 2.0. CgAsg1p changed the transcription level of several response genes，influencing stress regulation，redox process，transmembrane events，DNA replication，recombination and repair，etc. Besides，the mutant strain Cgasg1?驻 lost the ability of resisting oxidative stress and hyperosmotic stress. These results indicated that CgAsg1p is an essential transcription factor in C. glabrata for various stress responses.