Effects of Mixed Carbon Sources on Production and Antitumor Activity of Ganoderma lucidum Exopolysaccharides by Submerged Culture

Abstract:

To enhance production and activity of G. lucidum exopolysaccharides（EPS），a mixed carbon source containing glucose，galactose and mannose was applied and effects of mixed carbon sources on cell growth，EPS composition and antitumor activity were studied. Results showed that dry cell weight and EPS production were slightly affected by the ratio and type of monosaccharides. Anti-tumor activity analysis of G. lucidum EPS indicated that the raise of EPS concentration increased its biological activity. The inhibition reached up to 50%~60% with the ratio of glucose to galactose 1∶1 as carbon source，75%~85% with the ratio of glucose to galactose 1∶2，60%~65% with the ratio of glucose to mannose 1∶1，80%~85% with the ratio of glucose to mannose 1∶2 and 80%~85% with the same concentrations for three monosaccharides. The high ratio of galactose or mannose in culture medium was beneficial to the anti-tumor activity of EPS against the growth of A431and MDA-MB-231 cells.