Synthesis and Identification of Complete Antigen of Tulathromycin

Abstract:

The complete antigen of Tulathromycin was synthesized in this study. The Tulathromycin haptens were obtained by deriving the mycotoxins with succinic anhydride，introducing the free carboxyl group. The EDC method was applied to conjugate the Tulathromycin haptens to bovine serum albumin （BSA） as an artificial immunogen. The coating antigen Tulathromycin-OVA was achieved in the same way. The synthetic hapten was identified by LC-MS. Tulathromycin-BSA was characterized by fluorescence spectrometry and Bradford method. The antiserum titer was detected by indirect ELISA for BALB/c mice immunized with Tulathromycin-BSA. The conjugated-ratio of BSA was determined as 17∶1. The antiserum titer of the BALB/C mice reached to 1∶8 000 after the third Booster immunization. The complete antigens were successfully synthesized，which laid a foundation for the development of the Tulathromycin ELISA kit.