A Multiplex PCR Assay for the Rapid Screening of Genetically Modified Components in Food

Abstract:

Based on the DNA sequences of CaMV35S promoter，FaMV35S promoter，NOS terminator，bar gene，NPTII gene and CP4-EPSPS gene，a multiplex PCR assay for simultaneously screening the above six exogenous elements widely introduced into genetically modified organisms was established. The result showed that the highly efficient multiplex PCR system was obtained when the final primer concentrations were 0.2，0.2，0.3，0.3，0.2 and 0.2 μmol/L separately for them. The method could allow an accurate detection of six target elements from genetically modified maize，soybean，cotton and rapeseed，with the limit of detection of 0.1%. In conclusion，this multiplex PCR assay provides a highly effective approach to the screening of common transgenic components in foods and has high application value in the GMO detection.