Anti-Oxidative Activity of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein in vivo

Abstract:

The study was carried out to investigate the anti-oxidative effect of Microbial Transglutaminase Cross-Linked Fermentation Milk Protein（mTG-FPM） in aging mice induced by D-galactose（D-gal）. Sixty C57BL/6J female mice（12 w old）were divided into 6 groups including the control group，D-gal model group，D-gal-induced mice treated with FPM cross-linked mTG by 0 U，1 U and 3 U/g protein（as D-gal+0 UmTG-FMP，D-gal+1UmTG-FMP and D-gal+3 UmTG-FMP groups），at 1.5 g/kg bw via ig，and D-gal-induced mouse treated with vitamin E as the positive group at 100 mg/kg via ig，respectively. After continuous administration for 8 weeks，the anti-oxidative parameters（CAT，SOD，MDA GSH-Px） in liver，kidney tissues，and serum of mice in each group were determined. The results showed that mice induced by D-gal had lower CAT and GSH-Px activity in liver and serum，lower SOD activity of kidney and serum，and higher MDA content of kidney and serum，when compared with the normal mice（P < 0.01，P < 0.05）. Compared to the model and untreated FPM groups，FPM treated with mTG had higher CAT activities in liver tissue（3 U mTG treatment，P < 0.01） and in serum（1 U mTG treatment，P < 0.05） up to 11.14% and 35.57% respectively，whereas the content of MDA in kidney tissue（P < 0.01） in FPM treated with mTG was reduced 26.41%. The activity of GSH-Px in liver tissue was also sharply higher（22.36% increase） in D-gal+3 U mTG-FPM group than that of in FPM group untreated with mTG（P < 0.01）. It was concluded that the mTG Cross-linked effect in FPM can improve the anti-oxidative ability of aging mice induced by D-gal.