Methods and Optimization of High Throughput Sequencing Technologies on PCR Products

Abstract:

The development of the new generation sequencing technology extends the application field of gene sequencing. High throughput sequencing of PCR products has been widely used in functional gene screening，mutation and methylation detection of tumor related genes，etc. In the high throughput sequencing technology，database and computer sequencing experiments are the key factor to decide the quality of DNA data. Based on Illumina sequencing instruments，the sample preparation method and sequencing running for PCR products were optimized，the results make us clearly understand the primary features of high-throughput sequencing techniques，and provide us important references of sequencing methods for low diversity DNA samples to address biological questions of interest.