Cloning and Expression of Poly-γ-Glutamic Acid Synthase Gene of Bacillus methylotrophicus

Abstract:

The poly-γ-glutamic acid（γ-PGA） synthase gene pgsBCA of a glutamic acid-independent γ-PGA producing strain Bacillus methylotrophicus SK19.001 was cloned and expressed in the Escherichia coli. The pET-28a-pgs vector harboring pgsBCA genes were constructed and transformed into E.coli BL21. E. coli BL21 could synthesize γ-PGA when cultivated in the flask culture of LB plus glucose and L-glutamate，respectively. The pgsBCA genes were proved be essential for the synthesis of γ-PGA in B. methylotrophicus SK19.001. The blast result of deduced amino acid sequence from the pgsBCA genes in SK19.001 comparing other reported strains showed that the pgsB and pgsC gene were relatively conservative gene for pgsBCA.