Optimization of Mixed Enzymes to Improve the Quality of Tobacco Stems

Abstract:

The study aimed to optimize the hydrolysis parameters for mixed enzymes to improve the quality of tobacco stems and reduce the contents of macromolecules protein，pectin and cellulose. Results showed that acid protease was more sensitive to pH than pectease and cellulase but much less affected by temperature. The optimized parameters resulted in the better degradation of macromolecules with a total hydrolysis efficiency of 28.34% and significantly improved the sensory quality of tobacco stems. These indicated that hydrolysis rate and direction of tobacco stems could be changed according to industrial manufacturing，the requirement of sensory quality and the contents of macromolecules.