Abstract:The research aimed to study the impact of high-fat diet on thyroid hormone receptor and deiodinase in mice, as well as the protective effect of resveratrol. Four-week-old C57BL/6 male mice were fed normal diet, high-fat diet (HFD), or HFD supplemented with 0.1% resveratrol, respectively, metabolic experiments were conducted in the 6th week, the mice were sacrificed at the end of the 7th week and oxidative stress biomarker, blood lipid and thyroid hormone were then examined. The gene expressions of SIRT1, PGC1α, DIOI and TRβ were analyzed by RT-PCR. Compared with group C, body weight, blood lipid, MDA, T4 and FT4 levels of HFD mice were significantly increased (P < 0.05) whereas liver GSH/GSSG and T - AOC were remarkably reduced. Resveratrol obviously increased the expressions of DIOI, TR beta, SIRT1 and PGC1 alpha. Therefore, in a long term of high-fat feeding, the oxidative stress interfered with thyroid hormone but resveratrol could improve the metabolic abnormality by elevating the expressions of related genes SIRT1、PGC1α、DIOI and TRβ.
