Effects of Carbonated Drinks on Growth and Testosterone Secretion of Male Mice

Abstract:

One hundred male mice（20.84±2.45g） were randomly divided into five groups（n=20），COC-1 and COC-2 groups drank ad libitum 50% and 100% Coca-cola，PEP-1 and PEP-2 mice drank 50% and 100% Pepsi-cola and the control group（CG） drank tap water. Three mice selected randomly from each group were killed by cervical dislocation on the days 3，5，7，10，13 and 15；the serum was separated by centrifugal at 4000r/min for 10min；all visceral organs（heart，liver，spleen，lung，kidney，stomach and testis）were harvested aseptically；the concentration of serum testosterone was measured with the ELISA kit. On the 7th day body weights of four test groups were all higher than that of CG，especially for COC-2（P< 0.05）；on the 15th day the body weights of COC-2 and PEP-2 groups were higher than that of CG. Testis weights of COC-1，COC-2 and PEP-2 groups were significantly greater than groups CG and PEP-1 on the 7th day（P<0.05）；the stomach weight of PEP-2 was higher than other four groups on the 15th day. The concentration of serum testosterone in PEP-2 was significantly higher than those in COC-1 and CG（P < 0.05）；on the 10th day the concentrations in COC-2 and PEP-2 were dramatically higher than that in CG（P < 0.05）；on the 15th day the concentration in PEP-2 was remarkably higher than that in CG（P ＜0.05）. Thus，a short period of drinking of relatively high concentrations of Coca-cola and Pepsi-cola could promote the growth of male mice，enhance the testis development and increase the content of serum testosterone with no remarkable effects on visceral organs.