Abstract:From the second half of the 20th century, the invention of the protein and nucleic acid sequence detection technologies has induced an information explosion of the structure and function of biological macromolecules and the rational design of macromolecular sequence. Consequentially, an empire has been built to construct the functional tertiary structure of bio-macromolecules. In the 21st century, the innovative design wisdom in DNA technology began to spring up benefited from the interdisciplinary integration. The genome of a simple life such as virus and mycoplasma has been successfully recombined. Genome chassis has been designed with the construction of artificial evolution platform. A novel gene editing technique, named CRISPR, has been used as a powerful tool for genomic rectification. The technique of DNA origami improved the precise self-assembly of 3D DNA nano-materials. Moreover, the 3D bioinformatics database system can be constructed using the principle of stereoscopic constitution combined with the design method based on user experience.
