Rapid Detecion of Pantoea stewartii subsp. Stewartii Using Enzyme-Linked Immunoassay

Abstract:

A sensitive and rapid monoclonal antibody-based sandwich enzyme-linked immunosorbent assay（ELISA） was established to detect Pantoea stewartill subsp.Stewartii（Pss）. Three murine hybridomas（12B4/10B1/11C2） were obtained from the spleen cells fused produce. A pair of monoclonal antibodies（mAb） was selected for the sandwich ELISA method. The mAb 10B1 was conjugated with horseradish peroxidase（HRP） as the detection antibody and the mAb 11C2 was used as the capture antibody. The limit of detection of this method was 1.5×104 cfu/mL，and the linear range from 4.57×105 to 1.11×108 cfu/mL.