Immunomodulatory Effects of A Polysaccharide from Dictyophora indusiata on Macrophage

Abstract:

In order to investigate the immunomodulatory effects of a polysaccharide from Dictyophora indusiata （DI） on the immune function of RAW264.7 cells，the effects of DI on cell proliferation，phagocytosis of RAW264.7 cells，levels of NO，IL-1，IL-6 and TNF-α production were detected by MTT assay，neutral red test，Griess method，and ELISA，respectively. The mRNA expression level of iNOS，IL-1，IL-6 and TNF-α were also measured by reverse transcription polymerase chain reaction （RT-PCR）. The results indicated that DI contributed significantly to the proliferation of the macrophages and the phagocytic activity was at 25~200 μg/mL. In addition，the production of NO，IL-1，IL-6 and TNF-α in macrophages were increased. Further，the expression of iNOS，IL-1，IL-6 and TNF-α mRNA were significantly up-regulated. The DI played immunomodulatory effects on macrophage cell line RAW264.7.