Study on the Diversity of Intestinal Flora of Young Rural and Urban Residents in Wuxi

Abstract:

The gut microbial diversity of young people living in rural and urban Wuxi was studied by the combination of polymerase chain reaction（PCR） and denaturing gradient gel electrophoresis（DGGE）. Based on the DGGE profile，the similarity of volunteers was performed by analysis of clustering and PCA. On the other hand，the diversity was evaluated by the Shannon-Weine index，richness and eveness. The composition of gut microbiota was further characterized by sequencing of the common and special bands of the lanes in DGGE profile. Based on the PCR，the diversity of Lactobacillus and Bifidobacterium species in the gut of rural and urban volunteers was analyzed. The results showed that the structure of gut microbiota between rural and urban residents of Wuxi appeared a separated tendency，and the similarity of intestinal flora between them was lower than that of urban or rural residents alone. Furthermore，the diversity between them were not significantly different. The genus of Acinetobacter，Bifidobacterium，Staphylococcus，Lactobacillus，Clostridium and Ruminococcus were the common bacteria in the gut of young urban and rural residents. All kinds of bacteria of Lactobacillus（L.） and Bifidobacterium（B.） in the gut were not significantly different in rural and urban residents. And the species of L. plantarum，L. casei，L. salivarius，L. acidophilus，B. longum，B. breve，B. animalis and B. adolescentis were the predominant Lactobacillus and Bifidobacterium bacteria，respectively. On the base of the above comprehensive analysis，it can be preliminarily draw a conclusion that the diversity of gut microbiota was not significantly different between the young residents from rural and urban Wuxi.