Production of Pyrroloquinoline Quinone by Methylopila sp. YHT-1

Abstract:

A pyrroloquinoline quinone-producing strain YHT-1 was isolated from the soil using methanol as a single carbon source in the screening medium. Based on morphological, physiological and biochemical characteristics, as well as 16S rRNA sequences, YHT-1 was classified as a novel strain of the genus Methyolpila. After the preliminary optimization of fermentation conditions, it could produce 113.6 mg/L PQQ in a 3 L fermenter after 4 days fermentation. Separation and purification of PQQ by the anion-exchange chromatography on DEAE Sepharose F.F., the crude crystal was identified as PQQ according to HPLC and UV spectral analyses.