Purification and Characterization of Brown Pigment from Hazelnut Shells

Abstract:

On the basis of the preparatory purification of brown pigment from hazelnut shells with macroporous resin，the further purification with D101 resin was studied through single-factor and orthogonal experiments. The optimal parameters were found to be sample concentration of 22 mg/mL，velocity of 1.5 mL/min，eluting ethanol of 80% and velocity of 2.5 mL/min，which resulted in an increase of color value of the pigment from 44 to 82.6. The high temperature and the solution pH affected the stability of obtained brown pigment but light had no effect；fetal ions Fe3+，Cu2+，Al3+ and Zn2+ decreased its stability but Na+，Mg2+，Ca2+ and K+ had no effects；it efficiently colored starchy foods，eggs and meet.