Composition Analysis of Extracts Prepared by Solid-State Fermentation From Discarded Tobacco Leaves

Abstract:

The fermented extracts were prepared from discarded tobacco leaves using aerobic and anaerobic fermentation with high temperature and high moisture，followed by one-week aging after drying. The volatile components in the extracts were comparatively analyzed by headspace solid-phase micro-extraction（HS-SPME） associated with capillary gas chromatography-mass spectrometry（GC-MS）. The concentrated extracts were coated on film base for smoking quality analysis. There were totally 52 volatile aroma components detected in the aerobic fermentation tobacco extracts with a concentration of 43.38 μg/mL，and for extracts from anaerobic fermentation，there were 51 kinds of 40.16 μg/mL. For the control sample without fermentation，there were 34 components with concentration of 23.61 μg/mL. The contents of acids，esters，ketones，aldehydes，heterocycles were increased at different levels after fermentation. The significant effect of aroma enhancement and impurities removal confirmed that the smoking quality could be improved by fermentation treatments.