Structure Features and Monosaccharide Composition of the Intracellular Polysaccharide from Submerge-Cultured Ganoderma lucidum

Abstract:

The molecular weight and monosaccharide composition of intracellular polysaccharide from submerge-cultured Ganoderma lucidum（GLP） was investigated in this study，whose structure features was also thoroughly studied for the first time. This work could build a solid theoretical foundation for the further research on the biological activity and structure-activity relationship of GLP. The components of intracellular GLP were preliminary determined as glucose，galactose，mannose，arabinose and xylose with a molar ratio of 83.75∶4.76∶4.15∶2.04∶5.30 using monosaccharide composition analysis and spectrum analysis. The structure feature was confirmed with a backbone of 1，4-α-glucan and main terminal sugar residues of T-Glcp，and other branch residues containing galactose，mannose，xylose and arabinose by characterization of IR，GC-MS，HPAE-PAD and NMR，respectively.