Simultaneous Determination of 4 Flavonoid Glycosides in Indocalmus Leaf by HPLC

Abstract:

Indocalmus leaf is rich in flavonoids，which have various kinds of biological activities. Indocalmus leaves were collected from Songyang（Zhejiang province），Wuyi（Zhejiang province），Huangshan（Anhui province），Guangze（Fujian province） and Ningdu（Jiangxi province）. Samples were extracted by 50% ethanol and flavonoids including isoorientin，orientin，vitexin and isovitexin were then determined by high performance liquid chromatography（HPLC）. Results indicated the richness of favonoids in samples and the difference among isoorientin，orientin，vitexin and isovitexin in Indocalmus leaves from different regions，and thus provided a reference for deep research and development of Indocalmus leaf.