Study on Heterologous Expression of Burkholderia cepacia Lu10-1 Lipase

Abstract:

The lipase gene was isolated from Burkholderia cepacia Lu10-1 by PCR amplification and separately inserted into different vectors，which was then transformed to Escherichia coli，Pichia pastoris and Bacillus subtilis，respectively. The recombinant lipase was expressed as inclusion bodies in E. coli，while no expression of lipase was detected in P. pastoris. In B. subtilis，the lipase was extracellular expressed with an enzyme activity of 13.8 U/mL in the supernatant after fermentation. In addition，the optimization of culture condition for the recombinant B. subtilis was investigated in flask and 3 L stirred tank fermentor，respectively. After cultivation at 37 ℃ with the initial pH of 6.5 and TB used as the starting medium，the lipase activity in 3 L fermentor reached to 34.5 U/mL，a 4.2-fold to that of the original strain.