Construction and Analysis of the Transcriptome Before and After Infected by Penicillium in Hami Melon Fruit

Abstract:

Hami melon is famous with its unique flavor and rich nutrition. However，it is easily spoilt due to infected by Penicillium. The changes of transcriptome levels before and after infected by Penicillium in Hami melon were investigated by illumina paired-end sequencing. The results showed that the filtrated clean reads contained 4 947 555 420 nt nucleotides，further assembling the Clean Reads led to the product of 50 502 unigenes. A total of 71.28% unigenes were found in NR database using BLASTX comparison with an E-value cut-off of 1e-5，whereas 10 526 unigenes were still found to no significant matches to any known protein in NR，NT and Swiss-Prot database. These may be the specific genes leading to the difference between Hami melon and other species and need to be further verified. The unigene metabolic pathway analysis was conducted on the 39 976 unigenes through comparing to KEGG database and 128 metabolic pathways were mapped. Transcriptional analysis will greatly improve the understanding of postharvest physiology characteristics of Hami melon at the molecular level. It will provide a practical and effective model for the future research，such as exploring the expression and regulation of important genes related to Penicillium pathogen resistance.