Screening Mutation of High Glucose Oxidase Activity Using Atmospheric and Room Temperature Plasmas

Abstract:

Pre-cultured Aspergillus niger was treated using ARTP for the development of high glucose oxidase strain. Improved O-dianisidine color-developing circle method was used for preliminary screening and the mutant strains were re-screened by liquid state fermentation in flasks. Mutant strain T-6's enzyme activity increased from 45.6 U/mL to 85.3 U/mL and was selected for its high glucose oxidase-producing ability. Glucose oxidase activity reached 125.6 U/mL which was 175.4% higher than that of the original strain when the critical fermentation conditions，calcium carbonate and Tween-80 were optimized. The genetic expression of glucose oxidase was showed to be stable when the strain was cultured for 6 generations.