Expression and Application Analysis of Creatinase from Arthrobacter nicotianae

Abstract:

Creatine amidinohydrolase（CRE） is one of the key enzymes for clinical determination of creatinine in serum and urine. In this study，the gene encoding for CRE was successfully amplified from Arthrobacter nicotianae 23710 and functionally overexpressed in Escherichia coli BL21（DE3）. The recombinant CRE was purified through a series of operation including ammonium sulfate precipitation，anion exchange chromatography and gel filtration chromatography. The specific activity of the purified enzyme reached 20.25 U/mg. The recombinant enzyme shows excellent resistance to the chelating agent EDTA，the surfactants（Tween20 and Triton X-100） and the common preservative NaN3.