Enzymology and Immunogenicity of Uricase-Multivesicular Liposomes

Abstract:

This study aimed to investigate the enzymatic property and immunogenicity of uricase-multivesicular liposomes (UOMVLs). UOMVLs were prepared by a double emulsion method, and optimal temperatures, pH values, storage and thermal stabilities of UOMVLs and uricase (UOX) were determined. SD rats were immunized using UOMVLs as antigens, and their serum antibody titers were determined. Results showed that the optimal temperatures for UOMVLs and U0X were both 40 ℃, while the optimal pH values were 8.0 and 8.5, respectively. The stability of UOMVLs was significantly higher than that of U0X. The serum antibody titers of rats immunized with UOMVLs and UOX were 1∶500 and 1∶8 000, respectively. Therefore, the enzymatic property of UOMVLs was significantly better and the immunogenicity was obviously lower than UOX, which provided an experimental basis for the clinical application of UOX.