Study on Amylosynthease Activity and the Change of Starch Content in Transgenic Potato Tuber

Abstract:

The contents of amylose，amylopectin and total starch were determined via Dual-wavelength spectrophotometry and Anthrone colorimetry in transgenic potato tubers，respectively. Furthermore，activities of GBSS，SSS were measured. Independent samples t test were performed by using statistical SPSS software. The results showed that the contents of total starch and amylopectin in transgenic potato tubers（K5） were significantly higher than that in control group，while activities of GBSS，SSS and the amylose content of K5 decreased significantly. This indicated that synthase gene of transgenic potato was affected by RNA interference in some extent.