Expression of Recombinant Human Interleukin-29 Mutant in Pichia pastoris and Antitumor Analysis

Abstract:

Based on results of the bioinformatics analysis，a mutant of recombinant human interleukin 29（rhIL-29mut33） was constructed by site-directed mutagenesis and megaprimer PCR. Then，it was successfully expressed in Pichiapastoris GS115. The rhIL-29mut33 showed strong anti-proliferation effects on liver cancer cell BEL7402，colon cancer cell HCT8，and gastric cancer cell SGC7901 at different concentrations，and all of them showed stronger than that of wild type rhIL-29. At a high concentration of the rhIL-29mut33，the inhibition ratios of the three tumor cells were （31.76±2.87）%，（22.47±0.26）%，and （34.41±0.40）%，respectively. The superior biological activities of rhIL-29mut33，especially the antitumor activity，make it have great potential applications in the drug industry.