Study on the Preparation of Antioxidative Peptides from Enzymatic Hydrolysis of Laver and Their Comprehensive Utilization

Abstract:

This study integrate enzymatic reaction with several membrane technologies to construct a method for comprehensive utilization of antioxidant peptide and other active substances from laver. The hydrolysate of laver was separated and purified through ultrafiltration（10 kDa cut-off molecular weight） and nanofiltration（500 Da cut-off molecular weight）. After optimizing the operation parameters of membrane separation，recovery rates of antioxidant peptide，polysaccharide，dietary fiber，and peptide reached 73.1%，55%，85%，and 18%，respectively. The peptides from laver showed the DPPH scavenging activity. The DPPH scavenging activity of 0.5 mg/mL nanofiltration peptide solution was up to 68.5%. Furthermore，the optimum pH value of laver antioxidant peptide was 8.5. Meanwhile，these peptides showed a good stability，it retained about 83% relative scavenging activity of DPPH radical after heated at 80 ℃ for 6 h.