Isolation，Identification and Enzyme Purification of a SOD High Producing Strain of Lactic Acid Bacteria

Abstract:

One SOD high-producing strain was screened and identified from lactic acid bacteria. The SOD enzyme was purified. The microbial enrichment，Gram staining and Pyrogallol autoxidation determination method were used in the screening. Morphological characteristics，physiology and biochemistry experiments，16S-rDNA sequencing were used for the strain identification. The SOD enzyme was purified by ammonium sulfate precipitation，ultrafiltration，sephadex-G100，DEAE-Sepharose FF etc. A SOD high-producing strain （SD006） was obtained and identified as Lactobacillus plantrum. The activity of purified enzyme reached 37 959.2 U/g SD006 is a SOD high-producing probiotics strain of Lactobacillus plantrum. The SOD purified from Lactobacillus is valuable and can be developed and applied in the food industry.