Preparation and Characterization of Monoclonal Antibodies Against Listeria monocytogenes

Abstract:

In order to explore the specific monoclonal antibodies against Listeria monocytogenes for the immunological detection， irradiation life-style and thermal extinguishing method were used to produce Listeria nonocytogenes antibodies as the antigen，four hybridomas stably secreting McAbs to Listeria monocytogenes were developed. The results showed that fourhybridomas were successfully established，and the indirect ELISA titers of the ascites were 1：104～1：4×104. Their immune globulin subtypes were IgG1，Ig2a，Ig2b.Therefore，Listeria monocytogenes could be detected by a two-antibody-sandwich enzyme-linked immunosorbent assay （das-ELISA） formed by McAbs. The affinity constants （Kaffi） were from 1×107 L/mol to 1×1010 L/mol，which had no cross-reaction between the other compounds. The sensitivity of LM McAbs in the detection of antigen was as low as 1×103 CFU/mL. The high-titer，sensitive and specific anti-LM monoclonal antibodies were produced in this study，which makes it possible to develop immunoassay for detection of LM residues．