Mutation Breeding of Double Deficient Mutant Pichia pastoris X33 Strain

Abstract:

To promote the application of α-1, 6-mannose transferase (OCH1p) and α-1, 3-mannose transferase (ALG3p) double mutant strain of Pichia pastoris X33 (och1 alg3 mX33) as host for protein expression and to lay the foundation for the further glycosylation of the double mutant strain, this study wants to increase the growth rate as well as the ability to adapt of the double mutant strain through ultraviolet mutagenesis and Atmospheric and Room Temperature Plasma (ARTP) mutagenesis methods. At last, after four rounds of screening, a double mutant strain was found to be 15% increase in growth speed, the decrease in temperature sensitivity, and the improvement in cell survival rate.