Effect of Pachymaran to the Antioxidant Capacity and Bax, Bcl-2 Protein Expression of Renal Tissue in Mice with Type II Diabetes

Abstract:

The effect of polysaccharides from Wolfiporia cocos （Schw.） Ryv. & Cilbn（WRP）to the antioxidant capacity and protein Bax，Bcl-2 gene expression of renal tissues in mice with type II diabetes were studied. Non insulin dependent （type II） diabetes mellitus （NIDDM） was induced by the combination of high-carbohydrate/high-fat diet-fed and low dose of streptozotocin （STZ）. Mice were randomly assigned to four treatment groups：the control group，the NIDDM group，the group fed WRP diet and the group fed rosiglitazone diet. The antioxidant capacity and the expression of Bax and Bcl-2 in renal tissues of mice were investigated. The superoxide dismutase （SOD） and glutathione peroxidase （GPx） levels in NIDDM mice were increased while the malondialdehyde （MDA） level was decreased after fed with WRP，which indicated that WRP could protect kidney from the damage caused by diabetes by improving the antioxidation activity of kidney，reducing lipid peroxidation and preventing free radical induced oxidative stress. WRP could restrain excessive expression of bax gene in NIDDM mice and inhibit apoptosis of renal cells，which indicated preventive of diabetic nephropathy.