Study on the Effect of Electric Field on the Secondary Structure and Activity of Lipase

Abstract:

The changes in the secondary structures and activity of lipase after 5 min treatment using electric field with different strength were studied by FTIR（Fourier-transformation infrared spectroscopy）. The effect of electric field on the relative contents of the lipase secondary structures，i.e. α-helix，β-sheet，β-turn and random coil，varied within the range of 1.0 kV/cm to 6.0 kV/cm. Compared with the control groups，the relative contents of α-helix and β-sheet both decreased，namely，4.9%~10.9% for α-helix and 3.2%~22.6% for β-sheet respectively. The relative contents of β-turn and random coil increased instead，namely，12.5%~37.7% for β-turn and 3.1%~24% for random coil respectively. The most significant change of the relative contents for different secondary structure was observed at the 3.0 kV/cm electric field，which suggested the lipase activity was greatly improved after treatment by electric field. The improvement of the activity was related to the content increase of β-turn and random coil.