Isolation，Identification and Bacteriostasis of Spoilage Bacteria in Coating Solution of Paper-Making Reconstituted Tobacco Sheet

Abstract:

In this paper，the streak plate separation method was used to classify and identify the primary bacterial flora in the coating solution of paper-making reconstituted tobacco sheet according to colony shape，bacterial characteristics，physio-biochemical tests as well as 16S rDNA sequence analysis. The results indicated that the spoilage bacteria in the coating solution of paper-making reconstituted tobacco sheet were Bacillus pumilus，Bacillus licheniformis and Klebsiella pneumonia. Among them，Klebsiella pneumoniae was the main spoilage bacteria. Moreover，the antibacterial effects of five food preservatives[potassium sorbate，sodium diacetate，butylparaben sodium，EDTA-2Na and ε-polylysine（ε-PL）] on the spoilage bacteria were evaluated. The results revealed that antibacterial activities of these five preservatives ranked as follows：ε-PL> EDTA-2Na > sodium diacetate > potassium sorbate > butylparaben sodium. The minimal inhibitory concentration of ε-PL on Klebsiella pneumoniae was 0.075 mg/mL.