Purification and Characterization of β-Glucosidase from Aspergillus niger

Abstract:

Cellulose is one of the most popular-used biomass resource and widely applied in the bioethanol production，medicine，and food industry. The β-glucosidase（BGL） is of interest as the key enzyme in conversion of cellulose to glucose. In this study，glucosidase from Aspergillus niger in bean dregs broth was purified and characterized. The optimum temperature was 55 ℃，and the optimum pH was 2.0~2.5. BGL was stable at pH 2.0~7.5，The Km value was 8.4×10-4 mmol/L，the kcat value was 16 s-1 and the catalytic efficiency（kcat/Km） was 1.92×104 L/（mmol?s） in the optimum condition. This study could promote the theoretical research and the production and application of BGL.