A New Method for Agrobacterium-Mediated Transformation of the Mycelia of Pleurotus ostreatus

Abstract:

In this paper，we developed an easy and efficient method for Agrobacterium-mediated transformation of the mycelia of Pleurotus ostreatus. Using nested plates，the mycelia of Pleurotus ostreatus were grown on the inner plate. When the mycelia grew to the edge of the plate，the induced Agrobacterium tumeifaciens were mixed with co-cultivation medium（CCM） and then were added to the space between the inner and outer plate，the above mixtures further were co-cultivated with the growing mycelia. The average conversion rate of the transformations obtained by the usage of resistance screening to the hygromycin B is about 33%. The advantages of this method are simple operation，short term，and easy to expand the scale of experiments. The results may provide reference for the molecular breeding of Pleurotus ostreatus and the genetic transformation of the other filamentous fungi.