Design of Linker Peptides and Its Application in Fusion Protein

Abstract:

At present，the genetic engineering technology has become a hot research topic to modify enzyme molecule. Fusion enzyme technique based on the fusion protein design has been used in the construction of multi-functional enzymes and has shown the important potential application and theory values. As an indispensable component of recombinant fusion proteins，linker peptides have been shown to be important in the stability and bioactivity of fusion proteins. According to the latest research progress in recent years，the general properties of linkers derived from naturally-occurring multi-domain proteins，the classification and the advantageous of the linkers are summarized in this review. Finally，the key problems in the design of the linker and perspectives of this field were also discussed.