Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Dendrobium nobile Lindl.

Abstract:

The study aimed to optimize the ultrasonic-assisted extraction of polysaccharides from Dendrobium nobile Lindl. Using extraction rate as evaluation index，the polysaccharide content was assayed by the phenol-sulfuric method. Based on the orthogonal test，the optimal ultrasonic-assisted extraction conditions were：material-liquid ratio 1∶15（g/mL），extraction power 450 W，temperature 60 ℃ and time 50 min. With the optimal conditions，the extraction rate of polysaccharides was up to 3.11%.