Isolation, Identification and Bacteriostasis of Spoilage Bacteria in Pulp of Paper-Making Reconstituted Tobacco Sheet

Abstract:

In this paper，the streak plate separation method was used to classify and identify the primary bacterial flora in the pulp of paper-making reconstituted tobacco sheet according to colony shape，bacterial characteristics，physio-biochemical tests as well as 16S rDNA sequence analysis. The results indicated that the spoilage bacteria in the pulp of paper-making reconstituted tobacco sheet were Acinetobacter johnsonii and Pseudomonas sp. Besides，the antibacterial effects of five food preservatives（potassium sorbate，sodium diacetate，butylparaben sodium，EDTA-2Na and ε-polylysine（ε-PL）） on the spoilage bacteria were evaluated. The results revealed that these five preservatives could be ranked in decreasing order of antibacterial activity as follows：ε-PL> EDTA-2Na> butylparaben sodium > sodium diacetate > potassium sorbate. The minimal inhibitory concentrations of ε-PL to Acinetobacter johnsonii and Pseudomonas putida were 0.075 and 0.05 mg/mL，respectively.