Detection of Shark Derived Materials by Taqman Probe Fluorescent PCR

Abstract:

As the problem of adulteration of shark products worsens and the effective detection method lacks, this study designed a set of primers and probe based on the mitochondria DNA sequences of shark published in GenBank using the software of Primer Express 2.0, so as to establish a method of Taqman probe fluorescent PCR for the detection of shark derived materials. Results showed that 13 samples that had been identified as shark fins all displayed the amplified curves. The specificity of this method was good with all of 12 shark derived samples indicating the existence of shark DNA and with no amplification curves for the other 9 samples not derived from shark species. The detection is sensitive and the minimum detectable plasmid copy number was 10 copies/μL. It is also rapid and simple and the results have good repeatability and stability. Therefore, the method can be applied to the identification of shark derived products in marketplaces, such as fins.