Analysis of Large Molecular Weight Polysaccharide in the Fruiting Bodies of Ganoderma lucidum Cultivar ‘Hunonglingzhi 1’

Abstract:

The high molecular polysaccharide fraction was purified by precipitating a hot-water extract with 20%（V/V） ethanol from fruiting bodies of Ganoderma lucidum cultivar 'Hunonglingzhi 1'. The high performance size exclusion chromatography - multiple angle laser light scattering - refractive index detector（HPSEC-MALLS-RI） was adopted to determine the molecular characteristics of this fraction. Results indicated that the polysaccharide possessed high weight average molecular weight（Mw） of 2.28×106，small polydispersity of 1.14 and large gyration radius（Rg） of 150.1 nm，and existed with a rigid chain conformation in the liquid solution. Structural analysis showed that this polysaccharide was a β-（1，3）/（1，6）-linked D-glucan. Its content determined by HPLC in wood cultivated fruiting bodies was about 0.52 mg/g，which was significantly higher than that in bag cultivated pattern.