An Indirect Competitive Enzyme-Linked Immunosorbent Method for the Detection of Biotin

Abstract:

Different kinds of biotin immunogens were synthesized for mice immunization and the obtained monoclone antibody was used for the development of indirect competitive enzyme-linked immunosorbent assay. With the method of high specificity and sensitivity，the half inhibition concentration of biotin was 2.01 ng/mL and the detection limit was 0.32 ng/mL，and the biotin content in liquid milk and milk powder samples could be accurately detected. Compared with the national standard，the method was sufficiently stable，convenient，sensitive and accurate for the analysis of biotin in real samples.