Isolate, Identify and the Immune Improving Activity of Polysaccharides from Rape Bee Pollen

Abstract:

Rape bee pollen was powered and extracted by 95% ethanol under reflux，then extracting the filer residue by deionized water under 60 ℃. Adding 95% ethanol into the extract，rested and layered，then acquired the ethanol sediment of rape bee pollen. Isolating the ethanol sediment with DEAE-cellulose column，HW-55F chromatographic column，Sephacryl chromatographic column and Sepharose chromatographic column，acquired one main polysaccharide compound. Identifing the purity of this compound by Sephadex G-100 chromatographic column and ultraviolet spectral analysis，then using nuclear magnetic mathod to analyse the 1H-NMR、13C-NMR and 135DEPT-NMR of this compound，obtained its structural formula：HOCH2-[CHOH]4-COOH. Irrigating the immune suppression mice with this component of rape bee pollen，results showed that this component of rape bee pollen could improve the thymus index（P<0.01），spleen index（P<0.01） and serum IgG level（P<0.01） of immune suppression mice significantly.