Microwave-Assisted Extraction of Polysaccharides from Polyporus umbellatus Optimized by Box-Behnken Design-Response Surface Methodology

Abstract:

To optimize the microwave-assisted extraction technique of polysaccharide from Polyporus umbellatus，the effects of the liquid to solid ratio，pH，microwave power，extraction time，extraction number and their interaction on extaction were studied by Box-Behnken design. The predictive model and reliability were developed by Design Expert software and response surface analysis (RSM). The optimal extraction conditions were achieved and listed as follows:liquid-to-solid ratio 30∶1 mL/g，pH 6.6，microwave power 614 W，extraction time 2.5 mins，extraction number 3 times. Under these conditions，the extraction yield of polysaccharide was up to 6.75%. The extraction conditions achieved by RSM provided technical support for the industrial production of polysaccharide from Polyporus umbellatus.