Optimum Fermentation Condition of Production Lactose Oxidase by Raoultella terrigena Y20

Abstract:

The effects of fermentation conditions on lactose oxidase activity producing by Raoultella terrigena Y20，which was screened by our lab，were studied by single factor experiment and response surface methodology. The results showed that the four independent variables as initial pH value，fermentation time，inoculation amount and fermention temperature had significant and sequence effects on the lactose oxidase activity. The optimal fermention conditions were as follows， inoculum amount of 2%，initial pH at 7.2，fermented 25 h，temperature at 28 ℃. Under the optimal conditions，the lactose oxidase activity was 74.96 U/g，which was almost consistent with predicted value of 78.87 U/g.